prof. Ing. Vladimír Křen, DrSc.

Vedoucí vědecký pracovník

Publikace

Expanding Rutinosidase Versatility: Acylated Quercetin Glucopyranosides as Substrates

Nitrogen-Containing Flavonoids-Preparation and Biological Activity

Three-Step Enzymatic Remodeling of Chitin into Bioactive Chitooligomers

Glycopolymer Inhibitors of Galectin-3 Suppress the Markers of Tissue Remodeling in Pulmonary Hypertension

Diaminocyclopentane – l-Lysine Adducts: Potent and selective inhibitors of human O-GlcNAcase

Glycomimetic inhibitors of tandem-repeat galectins: Simple and efficient

Multimodal Carbon Monoxide Photorelease from Flavonoids

Farmaceutický přípravek pro léčbu lékově rezistentních bakteriálních infekcí

Highly functionalized diaminocyclopentanes: A new route to potent and selective inhibitors of human O-GlcNAcase

Galectin-targeting glycocalix[4]arenes can enter the cells

Rutinosidase and other diglycosidases: Rising stars in biotechnology

Oligosaccharide Ligands of Galectin-4 and Its Subunits: Multivalency Scores Highly

Recent trends in the treatment of cyanide-containing effluents: Comparison of different approaches

Hesperidin; Hesperetin; Rutinose; and Rhamnose Act as Skin Anti-Aging Agents

Metabolic profiling of silymarin constituents in urine and feces of healthy volunteers: A 90-day study

Glycocalix[4]arenes and their affinity to a library of galectins: the linker matters

Advanced high-affinity glycoconjugate ligands of galectins

Selectively Halogenated Flavonolignans—Preparation and Antibacterial Activity

Sulfation of Phenolic Acids: Chemoenzymatic vs. Chemical Synthesis

New Bacterial Aryl Sulfotransferases: Effective Tools for Sulfation of Polyphenols

Mutation Hotspot for Changing the Substrate Specificity of beta-N-Acetylhexosaminidase: A Library of GlcNAcases

Bacterial Aryl Sulfotransferases in Selective and Sustainable Sulfation of Biologically Active Compounds using Novel Sulfate Donors

Diaminocyclopentane-derived O-GlcNAcase inhibitors for combating tau hyperphosphorylation in Alzheimer’s disease

Methods of in vitro study of galectin-glycomaterial interaction

Carbohydrate-derived bicyclic selenazolines as new dual inhibitors (cholinesterases/OGA) against Alzheimer’s disease

Discovery of human hexosaminidase inhibitors by in situ screening of a library of mono- and divalent pyrrolidine iminosugars

Hypertransglycosylating Variants of the GH20 beta-N-Acetylhexosaminidase for the Synthesis of Chitooligomers

Silybin and its congeners: from traditional medicine to molecular effects

Interaction of Flavonoids with Zinc and Zinc-Containing Enzymes

Sulfated Phenolic Substances: Preparation and Optimized HPLC Analysis

Flavonolignans from silymarin modulate antibiotic resistance and virulence in Staphylococcus aureus

A Career in Biocatalysis: Kurt Faber

Engineered Glycosidases for the Synthesis of Analogs of Human Milk Oligosaccharides

Laccases and Tyrosinases in Organic Synthesis

Metabolism of Aldoximes and Nitriles in Plant-Associated Bacteria and Its Potential in Plant-Bacteria Interactions

Glycopolymers Decorated with 3-O-Substituted Thiodigalactosides as Potent Multivalent Inhibitors of Galectin-3

2-Acetamido-2-deoxy-d-glucono-1;5-lactone Sulfonylhydrazones: Synthesis and Evaluation as Inhibitors of Human OGA and HexB Enzymes

Preparation of Synthetic and Natural Derivatives of Flavonoids Using Suzuki-Miyaura Cross-Coupling Reaction

Reversible Lectin Binding to Glycan-Functionalized Graphene

Antioxidative Effect of Dietary Flavonoid Isoquercitrin on Human Ovarian Granulosa Cells HGL5 In Vitro

Silymarin dehydroflavonolignans chelate zinc and partially inhibit alcohol dehydrogenase

Exploration of GH94 Sequence Space for Enzyme Discovery Reveals a Novel Glucosylgalactose Phosphorylase Specificity

Mutagenesis of Catalytic Nucleophile of beta-Galactosidase Retains Residual Hydrolytic Activity and Affords a Transgalactosidase

Chirality Matters: Biological Activity of Optically Pure Silybin and Its Congeners

Metabolism of 2;3-Dehydrosilybin A and 2;3-Dehydrosilybin B: A Study with Human Hepatocytes and Recombinant UDP-Glucuronosyltransferases and Sulfotransferases

Continuous diastereomeric kinetic resolution—silybins a and b

Advanced glycosidases as ingenious biosynthetic instruments

Immunoprotective neo-glycoproteins: Chemoenzymatic synthesis of multivalent glycomimetics for inhibition of cancer-related galectin-3

Flavonolignans from silymarin do not intercalate into DNA: Rebuttal of data published in the paper J. Mol. Recognit. e2812 (2019)

Interaction between Galectin-3 and Integrins Mediates Cell-Matrix Adhesion in Endothelial Cells and Mesenchymal Stem Cells

Systematic review of pharmacokinetics and potential pharmacokinetic interactions of flavonolignans from silymarin

Cross-linking effects dictate the preference of galectins to bind LacNAc-decorated HPMA copolymers

Dehydroflavonolignans from Silymarin Potentiate Transition Metal Toxicity In Vitro but Are Protective for Isolated Erythrocytes Ex Vivo

Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation

Access to both anomers of rutinosyl azide using wild-type rutinosidase and its catalytic nucleophile mutant

Mild and Selective Method of Bromination of Flavonoids

Sulfated Metabolites of Luteolin; Myricetin; and Ampelopsin: Chemoenzymatic Preparation and Biophysical Properties

Transglycosidase activity of glycosynthase-type mutants of a fungal GH20 beta-N-acetylhexosaminidase

How Site-Directed Mutagenesis Boosted Selectivity of a Promiscuous Enzyme

Multidrug Resistance Modulation Activity of Silybin Derivatives and Their Anti-Inflammatory Potential

Dual Substrate Specificity of the Rutinosidase fromAspergillus nigerand the Role of Its Substrate Tunnel

Regioselective 3-O-Substitution of Unprotected Thiodigalactosides: Direct Route to Galectin Inhibitors

Mitochondrial Superoxide Production Decreases on Glucose-Stimulated Insulin Secretion in Pancreatic beta Cells Due to Decreasing Mitochondrial Matrix NADH/NAD(+) Ratio

Biocatalysis: ´A Jack of all Trades …´

Dual SMO/BRAF Inhibition by Flavonolignans from Silybum marianum

Glycopolymers for efficient inhibition of galectin-3: in vitro proof of efficacy using suppression of T lymphocyte apoptosis and tumor cell migration

Identification of UDP-glucuronosyltransferases involved in the metabolism of silymarin flavonolignans

Simple and Rapid HPLC Separation and Quantification of Flavonoid; Flavonolignans; and 2;3-Dehydroflavonolignans in Silymarin

Defying Multidrug Resistance! Modulation of Related Transporters by Flavonoids and Flavonolignans

A novel enzymatic tool for transferring GalNAc moiety onto challenging acceptors

Biotransformation of Silymarin Flavonolignans by Human Fecal Microbiota

Liquid chromatography-drift tube ion mobility-mass spectrometry as a new challenging tool for the separation and characterization of silymarin flavonolignans

Acceptor Specificity of ?-N-Acetylhexosaminidase from Talaromyces flavus: A Rational Explanation

The effect of silymarin flavonolignans and their sulfated conjugates on platelet aggregation and blood vessels ex vivo

The flavonoid degrading fungus Acremonium sp. DSM 24697 produces two diglycosidases with different specificities

Redox properties of individual quercetin moieties

In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin

2;3-Dehydroderivatives of Silymarin Flavonolignans: Prospective Natural Compounds for the Prevention of Chronic Diseases

Antioxidant; Anti-Inflammatory; and Multidrug Resistance Modulation Activity of Silychristin Derivatives

beta-N-Acetylhexosaminidases-the wizards of glycosylation

Identifying Efficient Clostridium difficile Toxin A Binders with a Multivalent Neo-Glycoprotein Glycan Library

Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements

Heterogenní způsob výroby rutinosy

Chemoenzymatic Synthesis and Radical Scavenging of Sulfated Hydroxytyrosol; Tyrosol; and Acetylated Derivatives

Preparation of Retinoyl-Flavonolignan Hybrids and Their Antioxidant Properties

Poor chemical and microbiological quality of the commercial milk thistle-based dietary supplements may account for their reported unsatisfactory and non-reproducible clinical outcomes

Potential of Mitochondria-Targeted Antioxidants to Prevent Oxidative Stress in Pancreatic beta-cells

Glycosidase-Catalyzed Synthesis of Glycosyl Esters and Phenolic Glycosides of Aromatic Acids

UV-Vis spectroelectrochemical investigation of hydroxy compounds complexation with copper

HPMA copolymers decorated with saccharide ligands: the architecture of spacer matters

On the reaction of polyphenols with copper dication

Selective Beta-N-acetylhexosaminidase from Aspergillus versicolor—a tool for producing bioactive carbohydrates

Enzyme-mediated transglycosylation of rutinose (6-O-alpha-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp. DSM 24697

Oxidation of flavonolignan silydianin to unexpected lactone-acid derivative

In vitro and in silico studies of the membrane permeability of natural flavonoids from Silybum marianum (L.) Gaertn. and their derivatives

Isolated Silymarin Flavonoids Increase Systemic and Hepatic Bilirubin Concentrations and Lower Lipoperoxidation in Mice

Bioproduction of Quercetin and Rutinose Catalyzed by Rutinosidase: Novel Concept of´Solid State Biocatalysis´

The beta-N-Acetylhexosaminidase in the Synthesis of Bioactive Glycans: Protein and Reaction Engineering

Galectin-Carbohydrate Interactions in Biomedicine and Biotechnology