Structural Characterization of Monoclonal Antibodies and Epitope Mapping by FFAP Footprinting 2024 Laboratoř strukturní biologie a buněčné signalizace Fojtík L.Kalaninová Z.Fiala J.Halada P.Chmelík J.Man P.Kukačka Z.Novák P.Analytical Chemistry. 2024; 96(19); 7386-739310.1021/acs.analchem.3c04161
Structural dynamics of Na+ and Ca2+ interactions with full-size mammalian NCX 2024 Laboratoř strukturní biologie a buněčné signalizace Giladi M.Fojtík L.Strauss T.Da'adoosh B.Hiller R.Man P.Khananshvili D.Communications Biology. 2024; 7(1); 46310.1038/s42003-024-06159-9
Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry 2023 Laboratoř strukturní biologie a buněčné signalizace Kalaninová Z.Fojtík L.Chmelík J.Novák P.Volný M.Man P.In: GEVAERT; K.; ed. Mass Spectrometry-Based Proteomics. New York: Humana; 2023; s. 303-334. Methods in Molecular Biology10.1007/978-1-0716-3457-8_17
Hydrogen/Deuterium Exchange Mass Spectrometry of Heme-Based Oxygen Sensor Proteins 2023 Laboratoř strukturní biologie a buněčné signalizace Vávra J.Sergunin A.Stráňava M.Kádek A.Shimitzu T.Man P.Martínková M.In: WEINERT; E.E.; ed. Oxygen Sensing. Methods and Protocols. New York: Humana; 2023; s. 99-12210.1007/978-1-0716-3080-8_8
A conserved tryptophan in the acylated segment of RTX toxins controls their ?inf2/inf integrin–independent cell penetration 2023 Laboratoř molekulární biologie bakteriálních patogenů Osičková A.Knoblochová Š.Bumba L.Man P.Kalaninová Z.Lepesheva A.Jurnečka D.Čížková M.Biedermannová L.Goldsmith J.Maynard J.McLellan J.Osička R.Šebo P.Mašín J.Journal of Biological Chemistry. 2023; 299(8); 10497810.1016/j.jbc.2023.104978
Counterintuitive structural and functional effects due to naturally occurring mutations targeting the active site of the disease-associated NQO1 enzyme 2023 Laboratoř strukturní biologie a buněčné signalizace Pacheco-Garcia J.Anoz-Carbonell E.Loginov D.Kavan D.Salido E.Man P.Medina M.Pey A.FEBS Journal. 2023; 290(7); 1855-187310.1111/febs.16677
Insights into the pathogenesis of primary hyperoxaluria type I from the structural dynamics of alanine:glyoxylate aminotransferase variants 2024 Laboratoř strukturní biologie a buněčné signalizace Vaňková P.Pacheco-Garcia J.Loginov D.Gomez-Mulas A.Kádek A.Martin-Garcia J.Salido E.Man P.Pey A.FEBS Letters. 2024; 598(4); 485-49910.1002/1873-3468.14800
A single evolutionarily divergent mutation determines the different FAD-binding affinities of human and rat NQO1 due to site-specific phosphorylation 2022 Laboratoř strukturní biologie a buněčné signalizace Luis Pacheco-Garcia J.Loginov D.Rizzuti B.Vaňková P.Neira J.Kavan D.Mesa-Torres N.Guzzi R.Man P.Pey A.FEBS Letters. 2022; 596(1); 29-4110.1002/1873-3468.14238
Allosteric Communication in the Multifunctional and Redox NQO1 Protein Studied by Cavity-Making Mutations 2022 Laboratoř strukturní biologie a buněčné signalizace Pacheco-Garcia J.Loginov D.Anoz-Carbonell E.Vaňková P.Palomino-Morales R.Salido E.Man P.Medina M.Naganathan A.Pey A.Antioxidants. 2022; 11(6); 111010.3390/antiox11061110
Different phenotypic outcome due to site-specific phosphorylation in the cancer-associated NQO1 enzyme studied by phosphomimetic mutations 2022 Laboratoř strukturní biologie a buněčné signalizace Pacheco-Garcia J.Anoz-Carbonell E.Loginov D.Vaňková P.Salido E.Man P.Medina M.Palomino-Morales R.Pey A.Archives of Biochemistry and Biophysics. 2022; 729(OCT 30 2022); 10939210.1016/j.abb.2022.109392
Loss of stability and unfolding cooperativity in hPGK1 upon gradual structural perturbation of its N-terminal domain hydrophobic core 2022 Laboratoř strukturní biologie a buněčné signalizace Luis Pacheco-Garcia J.Loginov D.Naganathan A.Vaňková P.Cano-Munoz M.Man P.Pey A.Scientific Reports. 2022; 12(1); 1720010.1038/s41598-022-22088-1
TTYH family members form tetrameric complexes at the cell membrane 2022 Laboratoř strukturní biologie a buněčné signalizace Melvin E.Kalaninová Z.Shlush E.Man P.Giladi M.Haitin Y.Communications Biology. 2022; 5(1); 88610.1038/s42003-022-03862-3
Structural basis for long-chain isoprenoid synthesis by cis-prenyltransferases 2022 Laboratoř strukturní biologie a buněčné signalizace Giladi M.Bar-El M.Vaňková P.Ferofontov A.Melvin E.Alkaderi S.Kavan D.Redko B.Haimov E.Wiener R.Man P.Haitin Y.Science Advances. 2022; 8(20); eabn117110.1126/sciadv.abn1171
Early modification of cytochrome c by hydrogen peroxide triggers its fast degradation 2021 Laboratoř strukturní biologie a buněčné signalizace Tomášková N.Novák P.Kožár T.Petrenčáková M.Jančura D.Yassaghi G.Man P.Sedlák E.International Journal of Biological Macromolecules. 2021; 174(MAR 31 2021); 413-42310.1016/j.ijbiomac.2021.01.189
Structural basis of the pleiotropic and specific phenotypic consequences of missense mutations in the multifunctional NAD(P)H:quinone oxidoreductase 1 and their pharmacological rescue 2021 Laboratoř strukturní biologie a buněčné signalizace Pacheco-Garcia J.Anoz-Carbonell E.Vaňková P.Kannan A.Palomino-Morales R.Mesa-Torres N.Salido E.Man P.Medina M.Naganathan A.Pey A.Redox Biology. 2021; 46(OCT 2021); 10211210.1016/j.redox.2021.102112
Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola 2021 Laboratoř strukturní biologie a buněčné signalizace Csarman F.Obermann T.Zanjko M.Man P.Halada P.Seiboth B.Ludwig R.Enzyme and Microbial Technology. 2021; 148(AUG 2021); 10980110.1016/j.enzmictec.2021.109801
Chimeric Cellobiose Dehydrogenases Reveal the Function of Cytochrome Domain Mobility for the Electron Transfer to Lytic Polysaccharide Monooxygenase 2021 Laboratoř strukturní biologie a buněčné signalizace Felice A.Schuster C.Kádek A.Filandr F.Laurent C.Scheiblbrandner S.Schwaiger L.Schachinger F.Kracher D.Sygmund C.Man P.Halada P.Oostenbrink C.Ludwig R.ACS Catalysis. 2021; 11(2); 517-53210.1021/acscatal.0c05294
Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry 2021 Laboratoř strukturní biologie a buněčné signalizace Filandrová R.Kavan D.Kádek A.Novák P.Man P.In: POTERSZMAN; Arnaud; ed. Multiprotein Complexes. Methods and Protocols. 2247. New York: Springer; 2021; s. 193-219. Methods in Molecular Biology10.1007/978-1-0716-1126-5_11
Different Reaction Specificities of F420H2-Dependent Reductases Facilitate Pyrrolobenzodiazepines and Lincomycin To Fit Their Biological Targets 2020 Laboratoř antibiotické rezistence a mikrobiálního metabolismuSteiningerová L.Kameník Z.Gažák R.Kadlčík S.Bashiri G.Man P.Kuzma M.Pavlíková M.Janata J.Journal of the American Chemical Society. 2020; 142(7); 3440-344810.1021/jacs.9b11234
HSPA1A conformational mutants reveal a conserved structural unit in Hsp70 proteins 2020 Laboratoř strukturní biologie a buněčné signalizace Vandová V.Vaňková P.Durech M.Houser J.Kavan D.Man P.Muller P.Trčka F.Biochimica et Biophysica Acta-General Subjects. 2020; 1864(1); 12945810.1016/j.bbagen.2019.129458
The interaction of the mitochondrial protein importer TOMM34 with HSP70 is regulated by TOMM34 phosphorylation and binding to 14-3-3 adaptors 2020 Laboratoř regulace genové expreseTrčka F.Durech M.Vaňková P.Vandová V.Šimončík O.Kavan D.Vojtěšek B.Muller P.Man P.Journal of Biological Chemistry. 2020; 295(27); 8928-894410.1074/jbc.RA120.012624
Bacteriocin ASM1 is an O/S-diglycosylated; plasmid-encoded homologue of glycocin F 2020 Laboratoř strukturní biologie a buněčné signalizace Main P.Hata T.Loo T.Man P.Novák P.Havlíček V.Norris G.Patchett M.FEBS Letters. 2020; 594(7); 1196-120610.1002/1873-3468.13708
Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation 2020 Laboratoř strukturní biologie a buněčné signalizace Petrenčáková M.Filandr F.Hovan A.Yassaghi G.Man P.Kožár T.Schwer M.Jančura D.Pluckthun A.Novák P.Miškovský P.Bano G.Sedlák E.Scientific Reports. 2020; 10(1); 411910.1038/s41598-020-60861-2
The H2O2-dependent activity of a fungal lytic polysaccharide monooxygenase investigated with a turbidimetric assay 2020 Laboratoř strukturní biologie a buněčné signalizace Filandr F.Man P.Halada P.Chang H.Ludwig R.Kracher D.Biotechnology for Biofuels. 2020; 13(1); 3710.1186/s13068-020-01673-4
Structural basis of heterotetrameric assembly and disease mutations in the human cis-prenyltransferase complex 2020 Laboratoř strukturní biologie a buněčné signalizace Bar-El Lisnyansky M.Vaňková P.Yeheskel A.Simhaev L.Engel H.Man P.Haitin Y.Giladi M.Nature Communications. 2020; 11(1); 527310.1038/s41467-020-18970-z
Conserved cysteine dioxidation enhances membrane interaction of human Cl(-)intracellular channel 5 2020 Laboratoř strukturní biologie a buněčné signalizace Ferofontov A.Vaňková P.Man P.Giladi M.Haitin Y.FASEB Journal. 2020; 34(8); 9925-994010.1096/fj.202000399R
Structural Dynamics of Lytic Polysaccharide Monooxygenase during Catalysis 2020 Laboratoř strukturní biologie a buněčné signalizace Filandr F.Kavan D.Kracher D.Laurent C.Ludwig R.Man P.Halada P.Biomolecules. 2020; 10(2); 24210.3390/biom10020242
14-3-3 protein binding blocks the dimerization interface of caspase-2 2020 Laboratoř strukturní biologie a buněčné signalizace Kalábová D.Filandr F.Alblová M.Petrvalská O.Horváth M.Man P.Obšil T.Obšilová V.FEBS Journal. 2020; 287(16); 3494-351010.1111/febs.15215
A Dynamic Core in Human NQO1 Controls the Functional and Stability Effects of Ligand Binding and Their Communication across the Enzyme Dimer 2019 Laboratoř strukturní biologie a buněčné signalizace Vaňková P.Salido E.Timson D.Man P.Pey A.Biomolecules. 2019; 9(11); 72810.3390/biom9110728
MS-Based Approaches Enable the Structural Characterization of Transcription Factor/DNA Response Element Complex 2019 Laboratoř charakterizace molekulární strukturySlavata L.Chmelík J.Kavan D.Filandrová R.Fiala J.Rosůlek M.Mrázek H.Kukačka Z.Vališ K.Man P.Miller M.McIntyre W.Fabris D.Novák P.Biomolecules. 2019; 9(10); 53510.3390/biom9100535
Recommendations for performing; interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments 2019 Laboratoř strukturní biologie a buněčné signalizace Masson G.Burke J.Ahn N.Anand G.Borchers C.Brier S.Bou-Assaf G.Engen J.Englander S.Faber J.Garlish R.Griffin P.Gross M.Guttman M.Hamuro Y.Heck A.Houde D.Iacob R.Jorgensen T.Kaltashov I.Klinman J.Konermann L.Man P.Mayne L.Pascal B.Reichmann D.Skehel M.Snijder J.Strutzenberg T.Underbakke E.Wagner C.Wales T.Walters B.Weis D.Wilson D.Wintrode P.Zhang Z.Zheng J.Schriemer D.Rand K.Nature Methods. 2019; 16(7); 595-60210.1038/s41592-019-0459-y
Improving the Sequence Coverage of Integral Membrane Proteins during Hydrogen/Deuterium Exchange Mass Spectrometry Experiments 2019 Laboratoř strukturní biologie a buněčné signalizace Moller I.Slivacka M.Hausner J.Nielsen A.Pospíšilová E.Merkle P.Lišková R.Polák M.Loland C.Kádek A.Man P.Rand K.Analytical Chemistry. 2019; 91(17); 10970-1097810.1021/acs.analchem.9b00973
A three-pronged ‚Pitchfork‘ strategy enables an extensive description of the human membrane proteome and the identification of missing proteins 2019 Laboratoř strukturní biologie a buněčné signalizace Vít O.Harant K.Klener P.Man P.Petrák J.Journal of Proteomics. 2019; 204(JUL 30); 10341110.1016/j.jprot.2019.103411
Ergochromes: Heretofore Neglected Side of Ergot Toxicity 2019 Laboratoř genetiky a metabolismu hubFlieger M.Stodůlková E.Wyka S.Černý J.Grobárová V.Píchová K.Novák P.Man P.Kuzma M.Cvak L.Broders K.Kolařík M.Toxins. 2019; 11(8); 43910.3390/toxins11080439
Thiopurine intolerance-causing mutations in NUDT15 induce temperature-dependent destabilization of the catalytic site 2019 Laboratoř strukturní biologie a buněčné signalizace Man P.Fábry M.Sieglová I.Kavan D.Novák P.Hnízda A.Biochimica Et Biophysica Acta-Proteins and Proteomics. 2019; 1867(4); 376-38110.1016/j.bbapap.2019.01.006
Human Stress-inducible Hsp70 Has a High Propensity to Form ATP-dependent Antiparallel Dimers That Are Differentially Regulated by Cochaperone Binding 2019 Laboratoř regulace genové expreseTrčka F.Ďurech M.Vaňková P.Chmelík J.Martínková V.Hausner J.Kádek A.Marcoux J.Klumpler T.Vojtěšek B.Müller P.Man P.Molecular & Cellular Proteomics. 2019; 18(2); 320-33710.1074/mcp.RA118.001044
