Two-dimensional acquisition for top-down native mass spectrometry of non-covalent protein complexes

Native mass spectrometry is a powerful structural biology tool to study protein complexes, their dynamics and composition. In its so-called top-down approach, it aims to link specific forms of proteins to higher assemblies, in which they function naturally and/or in a disease. The 2D-TOPMASS project will leverage the power and versatility offered by state-of-the-art Fourier transform ion cyclotron resonance mass spectrometry experimentally coupled to powerful ultraviolet and infrared laser ion activation techniques in combination with advanced two-dimensional acquisition schemes in order to push the boundaries of native top-down MS of large protein- and protein / nucleic acid complexes.