For access to the NGC Quest 10 Plus Chromatography System, please contact:
This instrument was purchased in 2025 through the Programme Johannes Amos Comenius – Renewal and modernization of the national infrastructure for translational medicine EATRIS-CZ (registration number CZ.02.01.01/00/23_015/0008208).
Specifications:
System Pump
- Composed of two dual pumps and a mixer, allowing isocratic or gradient elution
- Pressure limit: 25.2 MPa
- Mobile phase flow range: 0.001 – 10 ml/min
- System protection against overpressure
Sample Pump
- Flow rate: 0.01 – 100 ml/min
- Capability to connect multiple columns
- Real-time monitoring of column pressure drop
Detectors
- Detector capable of monitoring multiple wavelengths
- Conductivity detector with a range of 0.01 – 999 mS/cm
Valves
- Inlet valve with 8 inputs for the mobile phase
- Sample injection valve, including a sample injection kit
- Outlet valve – three-way
- Switching valve allowing selection between five columns
- Sample loops with volumes of 0.5 ml, 1 ml, 2 ml, and 5 ml
Fraction Collector
- Flexible – capable of collecting into various containers (from microtiter plates to test tubes, 0.1–50 ml)
- Controlled by the same software as the entire chromatography system
- Fraction collection based on volume or UV signal
Columns
- Column for size-exclusion chromatography, type 10/300 200
- Column for size-exclusion chromatography, type 10/300 75
- Column for size-exclusion chromatography, type 26/600 200 pg
- Column for size-exclusion chromatography, type 26/600 75 pg
Control Unit
- Equipped with control software and a 19″ monitor
- Control software allows:
- Full operation of the instrument
- Real-time visualization of measured data
- Evaluation of measured data
- Data export for further analysis
Cooling Cabinet
- Chromatography system installed in a cooling cabinet
- Operating temperature: +2 to +15 °C