For access to the Tecan Safire 2 Plate Reader, please contact:
Specifications:
- Plate formats: 6 to 1536 well plates
- Temperature control: Ambient + 4 °C to 42 °C
- Shaking: linear, orbital, 3 velocities
Fluorescence
- Wavelength selection: Monochromator
- Wavelength range: Excitation: 230 – 850 nm, emission: 280 – 850 nm
- Bandwidth: Adjustable from 2.5 nm – 10 nm below 280 nm, 5 nm – 20 nm else
- Wavelength accuracy: ± 1 nm
- Wavelength precision: ± 1 nm
Sensitivity – top reading
- 384 well plate: 0.2 fmol/well (fluorescein)
Sensitivity – bottom reading
- 384 well plate 0.6 fmol/well (fluorescein)
Time Resolved Fluorescence
- Sensitivity: 384 well plate: 60 amol/well (europium)
- Precision: 384 well plate: 3 mP standard deviation at 1nM (fluorescein)
Absorbance
- Wavelength range: 230 – 1000 nm
- Bandwidth: 2.5 nm below 280 nm, 5 nm else
- Wavelength accuracy: ± 1 nm
- Wavelength precision: ± 1 nm
- Measurement range: 0 – 4 OD
- Accuracy at 492 nm: 0 – 2 OD: ± 1% and 0.006 OD
- Precision at 492 nm: 0 – 2 OD: ± 0.5% and 0.005 OD
Measurement time (fluorescence/absorbance/polarization)
| Plate type | Fluorescence | Absorbance | FP |
|---|---|---|---|
| 384-well plate | 25 s | 25 s | 45 s |
| 1536-well plate | 37 s | 27 s | 108 s |
Additional features
- Interface connections: RS 232 serial & parallel port
- Software Driven: YES
- Power: 115-120V / 220-240V 50/60Hz
- Dimensions: 22″ x 21″ x 11″
- Weight: 88 lb