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Laboratory of Structural Biology and Cell Signaling 

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Structural Biology and Cell Signaling   

Novák LAB

Our group consists of a multidisciplinary team whose thematic focus is divided into two main directions. The structural biology group produces proteins by recombinant expression technologies and characterizes the protein or protein-ligand structures and their dynamics utilizing advanced mass spectrometric techniques, namely native mass spectrometry, hydrogen-deuterium exchange, protein chemical and radical footprinting, and protein cross-linking. Since those data are rather complex and there are few commercial solutions to interpret them, the laboratory is devoting a part of its effort to software development and code writing. The clinical proteomics unit examines the origin of various kinds of human pathologies from bacterial/viral infections through diabetes to kidney malfunction and dementia. The main strength of this team is the unique “know-how” to manufacture protein chips for immunoaffinity enrichment. These protein chips are fully compatible with mass spectrometric detection.

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Focus of research

Significant Discoveries, Awards and Achievements

Vladimír Hanuš Award 2023

2023 Zdeněk Kukačka

Fast Fluoroalkylation of Proteins Uncovers the Structure and Dynamics of Biological Macromolecules

Public services and cooperation

  • Structural characterization of the interaction of new drugs with their protein targets, validation of the production of biological therapeutics, quantification of mutant forms of proteins in clinical samples
  • Bruker Daltonics, Nested Therapeutics, and VantAI.ai
  • Yoni Haitin and Moshe Giladi, Tel Aviv University, Israel
  • Daniele Fabris, University of Connecticut, Storrs, Connecticut, U.S.A.
  • Charlotte Uetrecht, Centre for Structural Systems Biology, Hamburg, Germany

Our Publications

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Our Team

Meet the members of our Structural Biology and Cell Signaling Laboratory team
Novák Lab