Services – Mikrobiologický ústav AV ČR, v. v. i.

In which areas can we help you?

Cultivation of tumor and non-tumor cells of murine and human origin
Determination of cytotoxic and cytostatic effects of test substances on tumor and immune cells using proliferation assays ([³H]-thymidine incorporation assay, MTT assay)
Study of induction of apoptotic cell death (Annexin-V assay, determination of activated capsase-3)
Work with in vivo models – we have immunocompetent (Balb/c, C57BL/6), immunodeficient (NSG, Rag2^-/-, Nu/Nu, CD3ε^-/-) and TCR transgeneic (OT-I.Rag1^-/-.Ly5.1, OT-II.Rag1^-/-.Ly5.1) SPF mouse models. We primarily use these mice for testing the anti-tumor effects of polymeric chemotherapeutics or IL-2 immunocomplex-based immunotherapy
Tissue or peripheral blood processing and subsequent analysis of immune and non-immune cell populations by flow cytometry
Isolation of individual immune cell populations – MACS, FACS – sorting
Adoptive transfer of purified and CFSE- or CTV-labelled various subsets of T cells into congeneic acceptors with their following stimulation and analysis via FACS
Treg assay in vitro
Analysis of the gene expression on mRNA level in cell cultures or tissues by Real-time PCR
Detection of selected proteins using Western-blot or ELISA methods
Detection of cytokine-producing cells by ELISPOT

Economic Contract HS 520024 (contractual research) with SOTIO Biotech a.s.

Under this contract, the antitumor activity of the developed immunotherapeutic mSOT201 is being tested in mouse tumor models in vivo, and the study of immunotherapy based on the blockade of selected inhibitory molecules of T lymphocytes in mouse models of ovarian cancer is being conducted.

RNDr. Tomáš Etrych, DSc., Institute of Macromolecular Chemistry of the Czech Academy of Sciences
Prof. MUDr. Radek Špíšek, Ph.D., prof. PharmDr. Jitka Palich Fučíková Ph.D., Mgr. Irena Adkins, Ph.D., SOTIO a.s.
Doc. MUDr. Jan Bouček, Ph.D., 1st Faculty of Medicine, Charles University, and Motol University Hospital
Prof. MUDr. Aleksi Šedo, DrSc., Institute of Biochemistry and Experimental Oncology, 1st Faculty of Medicine, Charles University
Associate professor J.B. Spangler, Ph.D., Johns Hopkins University, USA
Professor K.C. Garcia, Ph.D.